Fig 1: Hepatocytes of PSC patients accumulate apical bulkheads and their absence correlates with the formation of aberrant liver cell rosettes A, BHepatocytes accumulate apical bulkheads in the liver tissue of primary sclerosing cholangitis (PSC) patients (N = 3 control patients and N = 4 PSC patients). (A) Overview images in liver tissue of control and PSC patients. Immunofluoresence for the apical membrane marker BSEP (magenta), F‐actin (green) and nuclei (grey). Scale bar 10 μm. (B) High‐resolution imaging of F‐actin (green) showing individual bile canaliculi in control or PSC liver tissue. Red arrowheads mark apical bulkheads. Scale bar 2 μm.CDifferent lumen morphologies of a typical bile canaliculus (left column) and bile duct (middle column) in control patients and rosette (right column) in PSC patients. Immunofluorescence for BSEP (magenta) and F‐actin (green). Shows also differences dependent on the imaging plane, i.e. transversal (top row) or longitudinal (bottom row) cut of the lumen. White dashed lines mark the lumen. Scale bar 2 μm.D3D reconstruction of segments from the bile canaliculi network in control patients showing narrow canaliculi (left), and in PSC patients, showing that liver cell rosettes form segments of epithelial tubes connected to the BC network (right).E3D reconstruction of an epithelial tube in PSC patients showing hepatocytes (grey) surrounding an individual segment of canalicular lumen (green). On the right is a transversal cross‐section through this tube revealing the typical morphology of the liver cell rosettes.FLiver cell rosettes in PSC patients are formed by hepatocyte‐like cells (N = 3 control patients and N = 4 PSC patients). Immunofluoresence for BSEP (magenta), F‐actin (green) and nuclei (grey) in healthy parenchyma (left) and bile duct (middle) in control patients and liver cell rosettes in PSC patients (right). Scale bar 10 μm. Source data are available online for this figure.
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